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KMID : 0390320160260010071
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Chungbuk Medical Journal
2016 Volume.26 No. 1 p.71 ~ p.78
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Effect of Melon Extracts on the Cellular Signaling of C2C12 Osteoblast Cells
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Jeon In-Sook
Kim Won-Dae
Choi Joong-Kook
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Abstract
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Purpose: Cucumis melo is a member of Cucurbitaceae family and is reported to have a broad spectrum of anti-oxidant, anti-inflammatory, anti-cancer activities. In this study, we investigated whether the water soluble fraction has an effect on the cellular signaling of C2C12 osteoblast cell line.
Materials and Methods: The pulp and seeds of Cucumis melo were extracted with PBS buffer at the RT or the whole fruit was squeezed out as extract (Eco-A), and tested in C2C12 murine cells for their effects on osteoblast differentiation process. Alkaline phosphatase assay and RT-PCR assay were carried out to study the expression of genes involved in Osteogenesis. 1 gram of Extramel-C powder was dissolved in 15ml PBS and was used as a control for the Melon extracts.
Results: Alkaine phosphatase activity, in C2C12 cells, appears to increase in the presence of the Cucumis melo extracts. Runx2 and Osterix, well known osteogenic markers, seem to increase better in response to Eco-A than to Extramel-C.
Conclusion: The aqueous extract of Cucumis melo appears to have an osteogenic effect during the differentiation process of C2C12 osteoblasts and further in vivo (mouse) study is necessary to confirm this result.
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KEYWORD
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Cucumis Melo, C2C12 Osteoblasts, Alkaline Phosphatase, Runx2, Osterix
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